pMIA3-Olônne
(Plasmid
#214447)
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PurposeExpresses eSpCas9 and gRNA targeting Olônne-18 GSH
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Depositing Lab
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Depositing OrganizationGenome Institute of Singapore, A*STAR Research Entities
Located in Singapore -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214447 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 214447-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 214447-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMIA3
- Backbone size w/o insert (bp) 9825
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeSpCas9-P2A-mRuby2-P2A-tp53dn
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gRNA/shRNA sequenceOlônne-18 GSH targeting gRNA (961rev)
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SpeciesSynthetic
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 214447-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 214447-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMIA3-Olônne was a gift from Roger Foo (Addgene plasmid # 214447 ; http://n2t.net/addgene:214447 ; RRID:Addgene_214447) -
For your References section:
Computationally defined and in vitro validated putative genomic safe harbour loci for transgene expression in human cells. Autio MI, Motakis E, Perrin A, Bin Amin T, Tiang Z, Do DV, Wang J, Tan JKM, Ding SSL, Tan WX, Lee CJM, Teo AKK, Foo R. Elife. 2024 Jan 2;13:e79592. doi: 10.7554/eLife.79592. 10.7554/eLife.79592 PubMed 38164941
Map uploaded by the depositor.
