pTOPO-25S rDNA
(Plasmid
#23996)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 23996 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepTOPO
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
-
Vector typeBacterial Cloning - TOPO
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsTOP10 or other E. coli strain
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert name25S rRNA from +5270 to oligo y
-
Alt name25S rDNA from +5270 to oligo y
-
SpeciesS. cerevisiae (budding yeast)
-
Insert Size (bp)200
-
Mutationnone
-
GenBank IDFN393078
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTOPO-25S rDNA was a gift from Susan Baserga (Addgene plasmid # 23996 ; http://n2t.net/addgene:23996 ; RRID:Addgene_23996) -
For your References section:
RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Gallagher JE, Dunbar DA, Granneman S, Mitchell BM, Osheim Y, Beyer AL, Baserga SJ. Genes Dev. 2004 Oct 15. 18(20):2506-17. 10.1101/gad.1226604 PubMed 15489292