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• Konermann et al
• pAAV_hSyn_TALEBB(NG)-NLS-SID4…
• Sequences

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Depositor Full Sequences (1)

Sequences from the depositing laboratory may be predicted or based on Sanger or NGS data. Addgene sequencing results may differ from the depositor-provided sequence.

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  Sequence > pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA sequence 5810 bps cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcgtcgggcgacctttggtcgcccgg cctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcac gcgtTGTACAgtgtctagactgcagagggccctgcgtatgagtgcaagtgggttttaggaccaggatgag gcggggtgggggtgcctacctgacgaccgaccccgacccactggacaagcacccaacccccattccccaa attgcgcatcccctatcagagagggggaggggaaacaggatgcggcgaggcgcgtgcgcactgccagctt cagcaccgcggacagtgccttcgcccccgcctggcggcgcgcgccaccgccgcctcagcactgaaggcgc gctgacgtcactcgccggtcccccgcaaactccccttcccggccaccttggtcgcgtccgcgccgccgcc ggcccagccggaccgcaccacgcgaggcgcgagataggggggcacgggcgcgaccatctgcgctgcggcg ccggcgactcagcgctgcctcagtctgcggtgggcagcggaggagtcgtgtcgtgcctgagagcgcagtc gagaaggatccGCCACCATGTACCCATACGATGTTCCAGATTACGCTGTAGATTTGAGAACTTTGGGATA TTCACAGCAGCAGCAGGAAAAGATCAAGCCCAAAGTGAGGTCGACAGTCGCGCAGCATCACGAAGCGCTG GTGGGTCATGGGTTTACACATGCCCACATCGTAGCCTTGTCGCAGCACCCTGCAGCCCTTGGCACGGTCG CCGTCAAGTACCAGGACATGATTGCGGCGTTGCCGGAAGCCACACATGAGGCGATCGTCGGTGTGGGGAA ACAGTGGAGCGGAGCCCGAGCGCTTGAGGCCCTGTTGACGGTCGCGGGAGAGCTGAGAGGGCCTCCCCTT CAGCTGGACACGGGCCAGTTGCTGAAGATCGCGAAGCGGGGAGGAGTCACGGCGGTCGAGGCGGTGCACG CGTGGCGCAATGCGCTCACGGGAGCACCCCTCAACCTGACAgagacccgtacgggtctcGACTCACGCCT GAGCAGGTAGTGGCTATTGCATCCAAtGGcGGGGGCAGACCCGCACTGGAGTCAATCGTGGCCCAGCTTT CGAGGCCGGACCCCGCGCTGGCCGCACTCACTAATGATCATCTTGTAGCGCTGGCCTGCCTCGGCGGACG ACCCGCCTTGGATGCGGTGAAGAAGGGGCTCCCGCACGCGCCTGCATTGATTAAGCGGACCAACAGAAGG ATTCCCGAGAGGACATCACATCGAGTGGCAgCTAGCAGCCCCAAGAAgAAGAGaAAGGTGGAGGCCAGCA TGAACATCCAGATGCTGCTGGAGGCCGCTGACTACCTGGAACGGAGAGAGCGCGAAGCCGAGCACGGATA TGCTTCAATGCTGCCCGGAAGCGGCATGAATATTCAGATGCTGCTGGAGGCTGCTGATTACCTGGAAAGG CGCGAACGGGAGGCCGAACATGGCTATGCTTCCATGCTGCCTGGGTCTGGAATGAATATCCAAATGCTGC TGGAGGCAGCCGATTACCTGGAACGGAGAGAAAGAGAAGCCGAGCACGGATACGCCAGCATGCTGCCAGG CAGCGGGATGAACATACAAATGCTGCTGGAGGCTGCCGATTACCTGGAGAGGCGCGAGAGAGAAGCTGAA CATGGCTATGCCTCTATGCTGCCCccatggGGCAGTGGAGAGGGCAGAGGAAGTCTGCTAACATGCGGTG ACGTCGAGGAGAATCCTGGCCCAATCGAGAAGTCCTTCGTAATAACAGATCCAAGACTGCCTGATTACCC CATCATTTTTGCCAGCGACGGTTTTCTGGAGCTCACCGAATATTCCAGGGAGGAAATCATGGGGAGAAAC GCCAGGTTCCTGCAAGGGCCTGAGACAGACCAGGCCACCGTTCAGAAGATTCGCGATGCTATCCGCGATC AGAGGGAAACCACTGTGCAGTTAATAAACTACACTAAGTCAGGCAAAAAGTTCTGGAATCTGCTTCATCT CCAACCTGTCCGGGATCGAAAGGGAGGGCTGCAATACTTCATCGGTGTGCAGCTGGTGGGAAGTGATCAT GTGTAAgaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactg gtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctat tgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttg tggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggca ttgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcat cgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcg gggaaatcatcgtcctttccttggctgctcgcctAtgttgccacctggattctgcgcgggacgtccttct gctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctct tccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccga gcgctgctcgagCTAGAGCTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTG CCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAA ATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGG AGGATTGGGAAGACAATAGCAGGCATGCTGGGGAggtaaccacgtgcggaccgagcggccgcaggaaccc ctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcg cccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcct gatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacg cgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccag cgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaa gctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttg atttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtc cacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattctttt gatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacg cgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgc atagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggca tccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccga aacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttc ttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacat tcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagta tgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctca cccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactg gatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcactttta aagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcataca ctattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagta agagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcg gaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttggga accggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacg ttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggagg cggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctgg agccggtgagcgtggaagccgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgta gttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcct cactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttca tttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgag ttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgc gcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagct accaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtag ccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttac cagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataa ggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaa ctgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatc cggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatcttta tagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagc ctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgt

Addgene-Verified Partial Sequences (3)

Addgene has verified portions of this plasmid. Results are shown below. Primers used for Sanger sequencing are indicated in the sequence header.

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  Sequence > hSyn1pro-F 5'-gcaagtgggttttaggacca-3' gggtgcctacctgAcGACcGACCCCGACCCACTGGACAAGCACCCAACCCCCATTCCCCAAATTGCGCAT CCCCTATCAGAGAGGGGGAGGGGAAACAGGATGCGGCGAGGCGCGTGCGCACTGCCAGCTTCAGCACCGC GGACAGTGCCTTCGCCCCCGCCTGGCGGCGCGCGCCACCGCCGCCTCAGCACTGAAGGCGCGCTGACGTC ACTCGCCGGTCCCCCGCAAACTCCCCTTCCCGGCCACCTTGGTCGCGTCCGCGCCGCCGCCGGCCCAGCC GGACCGCACCACGCGAGGCGCGAGATAGGGGGGCACGGGCGCGACCATCTGCGCTGCGGCGCCGGCGACT CAGCGCTGCCTCAGTCTGCGGTGGGCAGCGGAGGAGTCGTGTCGTGCCTGAGAGCGCAGTCGAGAAGGAT CCGCCACCATGTACCCATACGATGTTCCAGATTACGCTGTAGATTTGAGAACTTTGGGATATTCACAGCA GCAGCAGGAAAAGATCAAGCCCAAAGTGAGGTCGACAGTCGCGCAGCATCACGAAGCGCTGGTGGGTCAT GGGTTTACACATGCCCACATCGTAGCCTTGTCGCAGCACCCTGCAGCCCTTGGCACGGTCGCCGTCAAGT ACCAGGACATGATTGCGGCGTTGCCGGAAGCCACACATGAGGCGATCGTCGGTGTGGGGAAACAGTGGAG CGGAGCCCGAGCGCTTGAGGCCCTGTTGACGGTcGcGGGAGAGCTGAGAGGGCCTCCCcTTCAgCTGGAC ACGGGCCAgTTGCTGAAGATCGCGAAGCGGGGAGGAgTCACGGc
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  Sequence > SID4X-R 5'-CATTGAAGCATATCCGTGCTc-3' ctctccgttccaggtagtcAGCGGCCTCCAGCAGCATCTGGATGTTCATGCTGGCCTCCACCTTTCTCTT CTTCTTGGGGCTGCTAGCTGCCACTCGATGTGATGTCCTCTCGGGAATCCTTCTGTTGGTCCGCTTAATC AATGCAGGCGCGTGCGGGAGCCCCTTCTTCACCGCATCCAAGGCGGGTCGTCCGCCGAGGCAGGCCAGCG CTACAAGATGATCATTAGTGAGTGCGGCCAGCGCGGGGTCCGGCCTCGAAAGCTGGGCCACGATTGACTC CAGTGCGGGTCTGCCCCCGCCATTGGATGCAATAGCCACTACCTGCTCAGGCGTGAGTCGAGACCCGTAC GGGTCTCTGTCAGGTTGAGGGGTGCTCCCGTGAGCGCATTGCGCCACGCGTGCACCGCCTCGACCGCCGT GACTCCTCCCCGCTTCGCGATCTTCAGCAACTGGCCCGTGTCCAGCTGAAGGGGAGGCCCTCTCAGCTCT CCCGCGACCGTCAACAGGGCCTCAAGCGCTCGGGCTCCGCTCCACTGTTTCCCCACACCGACGATCGCCT CATGTGTGGCTTCCGGCAACGCCGCAATCATGTCCTGGTACTTGACGGCGACCGTGCCAAGGGCTGCAGG GTGCTGCGACAAGGCTACGATGTGGGCATGTGTAAACCCATGACCCACCAGCGCTTCGTGATGCTGCGCG ACTGTCGACCTCACTTTGGGCTTGATCTTTTCCTGCTGCTGCTGTGAATATCCCAAAGTTCTCAAATCTA CAGCGTAATCTGGAACATCGTATGGGTACATGGTGGCGGATCCTTCTCGACTGCGCTCTCAGGCACGACA CGACTCCTCCGCTGCCCACCGCAgAcTGAGGCAGCGCTGAgTCGCCGGCGCCGCAgCGCAgATGGTCGCG CCCGTGCCCCCCTATCTCGCGCCTCGCgTGGTGCGGTCCGGCTGGgCCGGCggcGGCgCGgAcgCgACCA AGGtGGCCGGgAAgGGGAgTTTGCGGGGGACcggcgagtgacgtcagcgcgccttcagtgctgaggcggc ggtggc
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  Sequence > WPRE-R 5'-CATAGCGTAAAAGGAGCAACA-3' CaGTcaATCTTTcaCaAATTTTGTAATCCAGAGGTTGATTATCGATAAGCTTGATATCGAATTCTTACAC ATGATCACTTCCCACCAGCTGCACACCGATGAAGTATTGCAGCCCTCCCTTTCGATCCCGGACAGGTTGG AGATGAAGCAGATTCCAGAACTTTTTGCCTGACTTAGTGTAGTTTATTAACTGCACAGTGGTTTCCCTCT GATCGCGGATAGCATCGCGAATCTTCTGAACGGTGGCCTGGTCTGTCTCAGGCCCTTGCAGGAACCTGGC GTTTCTCCCCATGATTTCCTCCCTGGAATATTCGGTGAGCTCCAGAAAACCGTCGCTGGCAAAAATGATG GGGTAATCAGGCAGTCTTGGATCTGTTATTACGAAGGACTTCTCGATTGGGCCAGGATTCTCCTCGACGT CACCGCATGTTAGCAGACTTCCTCTGCCCTCTCCACTGCCCCATGGGGGCAGCATAGAGGCATAGCCATG TTCAGCTTCTCTCTCGCGCCTCTCCAGGTAATCGGCAGCCTCCAGCAGCATTTGTATGTTCATCCCGCTG CCTGGCAGCATGCTGGCGTATCCGTGCTCGGCTTCTCTTTCTCTCCGTTCCAGGTAATCGGCTGCCTCCA GCAGCATTTGGATATTCATTCCAGACCCAGGCAGCATGGAAGCATAGCCATGTTCGGCCTCCCGTTCGCG CCTTTCCAGGTAATCAGCAGCCTCCAGCAGCATCTGAATATTCATGCCGCTTCCGGGCAGCATTGAAGCA TATCCGTGCTCGGCTTCGCGCTCTCTCCGTTCCAGGTAGTCAGCGGCCTCCAGCAGCATCTGGATGTTCA TGCTGGCCTCCACCTTTCTCTTCTTCTTGGGGCTGCTAGCTGCCACTCGATGTGATGTCCTCTCGGGAAT CCTTCTGTTGGTCCGCTTAATCAATGCAGGCGCGTGCgGGAgCCCCTTCTTCACCGCATCCAAGGCGGGT CGTCCGCCgAGGCAGGCCAgCGCTACAAgAtGATCATTAgTGAgTGCGGCCAGCgCGGGGTCCGGCCTCg AAAGCTGGGCCACgATTGACTCCAGTGcGGGTCtGCCcCCgCCATtGGAtGCa