Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pBUN501
(Plasmid #50582)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50582 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGreen-like binary vector
  • Backbone manufacturer
    Mullineaux Lab, see http://www.pgreen.ac.uk/
  • Vector type
    CRISPR ; Plant expression
  • Selectable markers
    Bar

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin and Spectinomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    zCas9D10A
  • Alt name
    Cas9
  • Species
    Synthetic
  • Mutation
    Cas9D10A nickase, was derived from the zCas9 and included in the toolkit to facilitate HR-based gene replacement or NHEJ-based insertion/deletion events (indels) through offset nicking under the precondition of the avoidance of off-target effects
  • Promoter Ubi1p
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on insert)
    • NLS (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    gRNA scaffold
  • Species
    Synthetic
  • Promoter AtU6-26p

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBUN501 was a gift from Qi-Jun Chen (Addgene plasmid # 50582 ; http://n2t.net/addgene:50582 ; RRID:Addgene_50582)
  • For your References section:

    A CRISPR/Cas9 toolkit for multiplex genome editing in plants. Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ. BMC Plant Biol. 2014 Nov 29;14(1):327. 10.1186/s12870-014-0327-y PubMed 25432517