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PurposeTo rescue the expression of DHHC5 in the knockdown cell lines
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Depositing Lab
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Depositing OrganizationUniversity of Texas Southwestern Medical Center
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 85812-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 85812-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCI-neo
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7800
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Modifications to backboneinserted 3x Flag tag
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZDHHC5
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SpeciesM. musculus (mouse)
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Insert Size (bp)2200
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GenBank IDNM_144887
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Entrez GeneZdhhc5 (a.k.a. 1110032A17Rik, AI451382, Zisp)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 85812-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 85812-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-neo-Flag-DHHC5 was a gift from Sandra Hofmann (Addgene plasmid # 85812 ; http://n2t.net/addgene:85812 ; RRID:Addgene_85812) -
For your References section:
Systematic siRNA Screen Unmasks NSCLC Growth Dependence by Palmitoyltransferase DHHC5. Tian H, Lu JY, Shao C, Huffman KE, Carstens RM, Larsen JE, Girard L, Liu H, Rodriguez-Canales J, Frenkel EP, Wistuba II, Minna JD, Hofmann SL. Mol Cancer Res. 2015 Apr;13(4):784-94. doi: 10.1158/1541-7786.MCR-14-0608. Epub 2015 Jan 8. 10.1158/1541-7786.MCR-14-0608 PubMed 25573953
