Firefly luciferase mutants as sensors of proteome stress.
Gupta R, Kasturi P, Bracher A, Loew C, Zheng M, Villella A, Garza D, Hartl FU, Raychaudhuri S
Nat Methods. 2011 Sep 4;8(10):879-84. doi: 10.1038/nmeth.1697.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 90170 | pCI-neo Fluc EGFP | EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins |
| 90171 | pCI-neo FlucSM EGFP | EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation |
| 90172 | pCI-neo FlucDM EGFP | EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation |