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An in vivo reporter for tracking lipid droplet dynamics in transparent zebrafish.

Lumaquin D, Johns E, Montal E, Weiss JM, Ola D, Abuhashem A, White RM
Elife. 2021 Jun 11;10. pii: 64744. doi: 10.7554/eLife.64744. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
172533pME-PLIN2-tdTOMATOPLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning
172534pDestTol2CG2--3.5ubb:plin2-tdtomatoZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker
172535pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomatoZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance

Antibodies from Article