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delta Zfp521
(Plasmid #104190)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104190 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 104190-D50 50 μg of DNA in Tris buffer $495
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DNA 104190-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCMV Sport6
  • Backbone size w/o insert (bp) 4400
  • Total vector size (bp) 7610
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zfp521
  • Alt name
    Evi3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3210
  • Mutation
    deletion of final 6 Zn fingers
  • Entrez Gene
    Zfp521 (a.k.a. B930086A16Rik, Evi3, Znf521)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer SP6 5'GATTTAGGTGACACTATAG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    IMAGE clone - nucleotide accession number BC021376

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

deletion of final six Zn fingers

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    delta Zfp521 was a gift from Kathryn Hentges (Addgene plasmid # 104190 ; http://n2t.net/addgene:104190 ; RRID:Addgene_104190)

  • For your References section:

    Zfp521 promotes B-cell viability and cyclin D1 gene expression in a B cell culture system. AlDallal S, Wolton K, Hentges KE. Leuk Res. 2016 Jul;46:10-7. doi: 10.1016/j.leukres.2016.03.013. Epub 2016 Apr 9. 10.1016/j.leukres.2016.03.013 PubMed 27107743
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