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p043 mTcf-4 B
(Plasmid #11031)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11031 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 11031-D50 50 μg of DNA in Tris buffer $495
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DNA 11031-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCS105
  • Backbone size w/o insert (bp) 4133
  • Vector type
    Mammalian Expression ; Xenopus, Avian, and Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tcf-4
  • Alt name
    Tcf7l2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Tcf7l2 (a.k.a. TCF4B, TCF4E, Tcf-4, Tcf4)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Positive clone encoding mTcf4 obtained from screening a mouse embryonic gut cDNA library with degenerate oligos complementary to the highly conserved HMG box of Tcf/LEF proteins.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p043 mTcf-4 B was a gift from Ramesh Shivdasani (Addgene plasmid # 11031 ; http://n2t.net/addgene:11031 ; RRID:Addgene_11031)

  • For your References section:

    A possible role for the high mobility group box transcription factor Tcf-4 in vertebrate gut epithelial cell differentiation. Lee YJ, Swencki B, Shoichet S, Shivdasani RA. J Biol Chem. 1999 Jan 15. 274(3):1566-72. 10.1074/jbc.274.3.1566 PubMed 9880534
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