RCAS(A) mdnTcf4Ben (CT#1130)
(Plasmid #14020)

• Depositing Lab: Cliff Tabin
• Publication: Kardon et al Dev Cell. 2003 Dec . 5(6):937-44.

Backbone

• Vector backbone: RCASBP(A)
• Backbone size w/o insert (bp): 11600
• Vector type: Retroviral ; avian expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Tcf4B
• Species: M. musculus (mouse)
• Mutation: Dominant negative Tcf4B, engrailed fusion
• Entrez Gene: Tcf7l2 (a.k.a. TCF4B, TCF4E, Tcf-4, Tcf4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: RCAS-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

RCAS dnTcf4en virus was constructed using the mouse Tcf4B splice variant (gift of G. Dressler), as the chick Tcf4B splice variant is expressed in the developing limbs. The dominant-negative (dn) form was created by deleting amino acids 2–50, resulting in the production of a putative dominant-negative protein unable to bind and be activated by beta-catenin, but able to bind DNA via its C-terminal HMG box. The repressor domain from the Drosophila engrailed gene was fused in-frame to the 3′end of the dnTcf4 construct in the shuttle vector pSlax13 and then subcloned into retroviral vector RCAS(BP) A.