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RCAS(A) mdnTcf4Ben (CT#1130)
(Plasmid #14020)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 14020 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    RCASBP(A)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tcf4B
  • Species
    M. musculus (mouse)
  • Mutation
    Dominant negative Tcf4B, engrailed fusion
  • Entrez Gene
    Tcf7l2 (a.k.a. TCF4B, TCF4E, Tcf-4, Tcf4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer RCAS-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

RCAS dnTcf4en virus was constructed using the mouse Tcf4B splice variant (gift of G. Dressler), as the chick Tcf4B splice variant is expressed in the developing limbs. The dominant-negative (dn) form was created by deleting amino acids 2–50, resulting in the production of a putative dominant-negative protein unable to bind and be activated by beta-catenin, but able to bind DNA via its C-terminal HMG box. The repressor domain from the Drosophila engrailed gene was fused in-frame to the 3′end of the dnTcf4 construct in the shuttle vector pSlax13 and then subcloned into retroviral vector RCAS(BP) A.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS(A) mdnTcf4Ben (CT#1130) was a gift from Cliff Tabin (Addgene plasmid # 14020 ; http://n2t.net/addgene:14020 ; RRID:Addgene_14020)
  • For your References section:

    A Tcf4-positive mesodermal population provides a prepattern for vertebrate limb muscle patterning. Kardon G, Harfe BD, Tabin CJ. Dev Cell. 2003 Dec . 5(6):937-44. 10.1016/S1534-5807(03)00360-5 PubMed 14667415