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p182 pK7-HDAC1 mut (GFP)
(Plasmid #11055)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11055 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 11055-D50 50 μg of DNA in Tris buffer $495
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DNA 11055-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pK7
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HDAC1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1300
  • Mutation
    Deletion of aa 1-56: deacetylase domain deletion
  • Entrez Gene
    Hdac1 (a.k.a. HD1, Hdac1-ps, MommeD5, RPD3)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing data finds T189A and D332G substitutions. These are not known to affect function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p182 pK7-HDAC1 mut (GFP) was a gift from Ramesh Shivdasani (Addgene plasmid # 11055 ; http://n2t.net/addgene:11055 ; RRID:Addgene_11055)

  • For your References section:

    Regulation of mammalian epithelial differentiation and intestine development by class I histone deacetylases. Tou L, Liu Q, Shivdasani RA. Mol Cell Biol. 2004 Apr . 24(8):3132-9. 10.1128/MCB.24.8.3132-3139.2004 PubMed 15060137
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