Addgene - HDAC1 Flag Plasmid Data

Plasmid 13820: HDAC1 Flag

Gene/insert name:
HDAC1
Alt name:
Histone Deacetylase 1
Insert size:
1600
Species:
H. sapiens (human)
GenBank ID:
NM_004964
Entrez Gene:
HDAC1 (RP4-811H24.2, GON-10, HD1, RPD3, RPD3L1)
Fusion protein or tag:
flag
Terminal:
C terminal on backbone
Vector backbone:
pcDNA3.1 +
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
BGH Reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Eric Verdin
Terms and Licenses:
MTA

Comments:

cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Characterization of a human RPD3 ortholog, HDAC3. Emiliani et al (Proc Natl Acad Sci U S A. 1998 Mar 17. 95(6):2795-800. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13820" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with