HDAC1 Flag
(Plasmid #13820)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA3.1 +
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    HDAC1
  • Alt name
    Histone Deacetylase 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • GenBank ID
    NM_004964
  • Entrez Gene
    HDAC1 (a.k.a. RP4-811H24.2, GON-10, HD1, RPD3, RPD3L1)
  • Tag / Fusion Protein
    • flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HDAC1 Flag was a gift from Eric Verdin (Addgene plasmid # 13820)
  • For your References section:

    Characterization of a human RPD3 ortholog, HDAC3. Emiliani S, Fischle W, Van Lint C, Al-Abed Y, Verdin E. Proc Natl Acad Sci U S A. 1998 Mar 17. 95(6):2795-800. 10.1073/pnas.95.6.2795 PubMed 9501169