pcDNA6 MCV LTco S239A
(Plasmid #112194)

• Purpose: Expression of MCPyV LT mutant (Ser 239 to Ala)
• Depositing Lab: Patrick Moore
• Depositing Organization: University of Pittsburgh (University, School of Medicine, and Cancer Institute)
  Located in the United States of America
• Publication: Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4040-E4047. doi: 10.1073/pnas.1703879114. Epub 2017 May 1.

Backbone

• Vector backbone: pcDNA6.V5.HisB
• Backbone manufacturer: Invirtogen
• Backbone size w/o insert (bp): 4985
• Total vector size (bp): 7549
• Modifications to backbone: pcDNA6.V5.HisB vector was modified with NheI and PmeI restriction digestion enzymes to cut out MCS, V5 and His tag sites. New multiple cloning sites using annealed primer pair were ligated back into the vector. The newly generated cloning site comprises: NheI, KpnI, EcoRV, XhoI and SacII. Codon optimized Large T was cloned between EcoRV and XhoI restriction sites.
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MCPyV LT codon optimized
• Alt name: MCV LT
• Alt name: MCV large T antigen
• Alt name: Merkel Cell Polyomavirus large T antigen
• Species: Merkel cell polyomavirus
• Insert Size (bp): 2564
• GenBank ID: AEM01097.1
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHr

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 112194-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 112194-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.