Addgene - MSCV-N MCV ER Plasmid Data

Plasmid 37860: MSCV-N MCV ER

Gene/insert name:
MCV ER
Alt name:
Merkel Cell Small T
Alt name:
Merkel Cell Large T
Alt name:
MCPyV_gp1 and MCPyV_gp2
Insert size:
2885
Species:
Human Merkel Cell Virus
GenBank ID:
Entrez Gene:
MCPyV_gp2 (MCPyV_gp2)
MCPyV_gp1 (MCPyV_gp1)
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Vector backbone:
MSCV-N FLAGHA
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
8162
Promoter:
PKG
Cloning method:
Gateway Cloning
5' sequencing primer:
LXSN 5' List of Sequencing Primers
3' sequencing primer:
MSCV-N seq. rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
James DeCaprio lab
Sequence: View sequences (2)
Principal Investigator:
Karl Munger
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins. Rozenblatt-Rosen et al (Nature. 2012 Jul 26;487(7408):491-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37860" in your Materials and Methods section.

Price: $65.00

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