pRYG
(Plasmid
#113642)
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PurposePositive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.
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Depositing Lab
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Depositing OrganizationUniversity of Texas at Austin
Located in United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113642 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 113642-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 113642-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepQE-80L. ColE1
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDO NOT USE CAM/CAT; This resistance gene is not functional
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemRFP1-linker-sfGFP
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DNA (Catalog # 113642-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 113642-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRYG was a gift from Jeffrey Barrick (Addgene plasmid # 113642 ; http://n2t.net/addgene:113642 ; RRID:Addgene_113642) -
For your References section:
Rapid and inexpensive evaluation of nonstandard amino acid incorporation in Escherichia coli. Monk JW, Leonard SP, Brown CW, Hammerling MJ, Mortensen C, Gutierrez AE, Shin NY, Watkins E, Mishler DM, Barrick JE. ACS Synth Biol. 2016 Sep 20. 10.1021/acssynbio.6b00192 PubMed 27648665
