phRL TK 5BoxB sp 36
(Plasmid
#115365)
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PurposeExpression vector to produce humanized Renilla luciferase with 5BoxB sequences in the 3'UTR
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Depositing Labs
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Depositing OrganizationFriedrich Miescher Institute for Biomedical Research
Located in Switzerland -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 115365 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 115365-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 115365-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTK
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Backbone manufacturerThermoFisher
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Vector typeMammalian Expression, Luciferase
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRenilla Luciferase
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Alt name5BoxB
- Promoter TK
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer use reverse primer
- 3′ sequencing primer EBV-rev [5'-GTGGTTTGTCCAAACTCATC-3']
- (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
clone number 122. BoxB repeat sequence: gggccctgaagaagggccc
DNA (Catalog # 115365-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 115365-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
phRL TK 5BoxB sp 36 was a gift from Witold Filipowicz & Ramesh Pillai (Addgene plasmid # 115365 ; http://n2t.net/addgene:115365 ; RRID:Addgene_115365) -
For your References section:
Tethering of human Ago proteins to mRNA mimics the miRNA-mediated repression of protein synthesis. Pillai RS, Artus CG, Filipowicz W. RNA. 2004 Oct . 10(10):1518-25. 10.1261/rna.7131604 PubMed 15337849
