EGFP-hAgo2
(Plasmid #21981)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    EGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Argonaute 2
  • Alt name
    eiF2C2, Ago2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2580
  • Entrez Gene
    AGO2 (a.k.a. EIF2C2, Q10)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-hAgo2 was a gift from Phil Sharp (Addgene plasmid # 21981)
  • For your References section:

    Quantitative analysis of Argonaute protein reveals microRNA-dependent localization to stress granules. Leung AK, Calabrese JM, Sharp PA. Proc Natl Acad Sci U S A. 2006 Nov 28. 103(48):18125-30. 10.1073/pnas.0608845103 PubMed 17116888