|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19881||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5421
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameDicer 1, ribonuclease type III
Alt nameDCR1; Dicer; HERNA; KIAA0928
SpeciesH. sapiens (human)
Insert Size (bp)5772
Entrez GeneDICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2)
/ Fusion Protein
- FLAG/HA (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV (Common Sequencing Primers)
The Dicer insert contains an N1659Y mutation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFRT/TO/FLAG/HA-DEST DICER was a gift from Thomas Tuschl (Addgene plasmid # 19881 ; http://n2t.net/addgene:19881 ; RRID:Addgene_19881)
For your References section:Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs. Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, Ojo T, Hafner M, Zavolan M, Tuschl T. RNA. 2008 Oct 31. ():. 10.1261/rna.1351608 PubMed 18978028