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pDESTmycDICER
(Plasmid #19873)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19873 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dicer 1, ribonuclease type III
  • Alt name
    DCR1, Dicer, HERNA, KIAA0928
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5772
  • GenBank ID
    NM_177438
  • Entrez Gene
    DICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2)
  • Tags / Fusion Proteins
    • Myc (N terminal on backbone)
    • His (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Note that there are some discrepancies between Addgene's quality control sequence and the depositor sequence. Addgene's sequence aligns to human DICER better than the depositor sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDESTmycDICER was a gift from Thomas Tuschl (Addgene plasmid # 19873 ; http://n2t.net/addgene:19873 ; RRID:Addgene_19873)
  • For your References section:

    Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs. Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, Ojo T, Hafner M, Zavolan M, Tuschl T. RNA. 2008 Oct 31. ():. 10.1261/rna.1351608 PubMed 18978028