pDESTmycDICER
(Plasmid #19873)

• Depositing Lab: Thomas Tuschl
• Publication: Landthaler et al RNA. 2008 Oct 31. ():.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	19873	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pDESTmyc
  (Search Vector Database)
• Backbone size w/o insert (bp): 5862
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dicer 1, ribonuclease type III
• Alt name: DCR1, Dicer, HERNA, KIAA0928
• Species: H. sapiens (human)
• Insert Size (bp): 5772
• GenBank ID: NM_177438
• Entrez Gene: DICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2)
• Tags / Fusion Proteins:
  • Myc (N terminal on backbone)
  • His (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV

Resource Information

• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 11 References

Depositor Comments

Note that there are some discrepancies between Addgene's quality control sequence and the depositor sequence. Addgene's sequence aligns to human DICER better than the depositor sequence.