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pIRESneo-FLAG/HA Ago2 corrected
(Plasmid #10822)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10822 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIRESneo
  • Backbone size w/o insert (bp) 5310
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Argonaute 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2580
  • Mutation
    3 silent mutations in orf
  • GenBank ID
    NM_012154
  • Entrez Gene
    AGO2 (a.k.a. CASC7, EIF2C2, LINC00980, PPD, Q10)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ggtaccgagctcggatcgat
  • 3′ sequencing primer agctgttggggtgagtactcc
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRESneo-FLAG/HA Ago2 corrected was a gift from Thomas Tuschl (Addgene plasmid # 10822)
  • For your References section:

    Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T. Mol Cell. 2004 Jul 23. 15(2):185-97. 10.1016/j.molcel.2004.07.007 PubMed 15260970