Addgene - pIRESneo-FLAG/HA Ago1 Plasmid Data

Plasmid 10820: pIRESneo-FLAG/HA Ago1

Gene/insert name:
Argonaute 1
Insert size:
2571
Species:
H. sapiens (human)
GenBank ID:
NM_012199
Entrez Gene:
EIF2C1 (RP4-789D17.1, AGO1, EIF2C, GERP95, Q99)
Fusion protein or tag:
FLAG
Terminal:
N terminal on insert
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pIRESneo
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5313
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
ggtaccgagctcggatcgat List of Sequencing Primers
3' sequencing primer:
agctgttggggtgagtactcc
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Thomas Tuschl
Terms and Licenses:
MTA

Comments:

A small insertion in the 5'UTR should not affect the expressed peptide.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Meister et al (Mol Cell. 2004 Jul 23. 15(2):185-97. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10820" in your Materials and Methods section.