pVenusCfC
(Plasmid
#128567)
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PurposeProtein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.
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Depositing Lab
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Depositing OrganizationRudjer Boskovic Institute
Located in Croatia -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128567 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 128567-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 128567-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepDEST15
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Backbone manufacturerThermoFisher Scientific
- Backbone size w/o insert (bp) 7470
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVenus
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Alt nameSplit-GFP
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Alt nameSplit Venus
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SpeciesAequorea victoria
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Insert Size (bp)474
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer unknown
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Tiago Fleming Outeiro; University Medical Center Göttingen Department of Experimental Neurodegeneration
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' cloning site: AgeI (unknown if destroyed), 3' cloning site: MluI (unknown if destroyed)
DNA (Catalog # 128567-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 128567-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVenusCfC was a gift from Oliver Vugrek (Addgene plasmid # 128567 ; http://n2t.net/addgene:128567 ; RRID:Addgene_128567) -
For your References section:
Combining Unique Multiplex Gateway Cloning and Bimolecular Fluorescence Complementation (BiFC) for High-Throughput Screening of Protein-Protein Interactions. Lepur A, Kovacevic L, Beluzic R, Vugrek O. J Biomol Screen. 2016 Dec;21(10):1100-1111. doi: 10.1177/1087057116659438. Epub 2016 Jul 28. 10.1177/1087057116659438 PubMed 27455993
