pEGFP-CD86-mApple
(Plasmid
#133860)
-
PurposeMammalian expression of CD86 N-terminally tagged with EGFP and C-terminally tagged with mApple
-
Depositing Lab
-
Depositing OrganizationWashington University in Saint Louis
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133860 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 133860-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 133860-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backboneN1
-
Backbone manufacturerClontech
- Total vector size (bp) 6509
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameCD86
-
Alt nameB7-2
-
SpeciesH. sapiens (human)
-
Entrez GeneCD86 (a.k.a. B7-2, B7.2, B70, CD28LG2, LAB72)
-
Tags
/ Fusion Proteins
- mApple (C terminal on insert)
- EGFP (N terminal on insert)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byMike Heilemann via Addgene #98284
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 133860-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 133860-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEGFP-CD86-mApple was a gift from Dave Piston (Addgene plasmid # 133860 ; http://n2t.net/addgene:133860 ; RRID:Addgene_133860) -
For your References section:
Two-Color Spatial Cumulant Analysis Detects Heteromeric Interactions between Membrane Proteins. Foust DJ, Godin AG, Ustione A, Wiseman PW, Piston DW. Biophys J. 2019 Nov 5;117(9):1764-1777. doi: 10.1016/j.bpj.2019.09.028. Epub 2019 Sep 28. 10.1016/j.bpj.2019.09.028 PubMed 31606123
Map uploaded by the depositor.
