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pirespuro2-CTLA4-mEos2
(Plasmid #98285)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 98285 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CTLA4
  • Species
    H. sapiens (human)
  • Entrez Gene
    CTLA4 (a.k.a. ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEos2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

From depositor: The last 23 amino acids of CTLA-4 were
removed by PCR to reduce the internalization of the receptor and
concentrate it at the plasma membrane.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pirespuro2-CTLA4-mEos2 was a gift from Mike Heilemann (Addgene plasmid # 98285 ; http://n2t.net/addgene:98285 ; RRID:Addgene_98285)
  • For your References section:

    One, two or three? Probing the stoichiometry of membrane proteins by single-molecule localization microscopy. Fricke F, Beaudouin J, Eils R, Heilemann M. Sci Rep. 2015 Sep 11;5:14072. doi: 10.1038/srep14072. 10.1038/srep14072 PubMed 26358640