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pcDNA3.1-SARS-Spike
(Plasmid #145031)

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Ordering

Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 145031 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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DNA 145031-D50 50 μg of DNA in Tris buffer $495 *
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DNA 145031-D100 100 μg of DNA in Tris buffer $585 *
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 9300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV Spike
  • Species
    Synthetic
  • Insert Size (bp)
    3840
  • Entrez Gene
    S (a.k.a. sars2, E2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • C9 (C terminal on insert)
    • CD5 signal peptide (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7-F
  • 3′ sequencing primer BGH-R
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that immediate availability of this material is restricted to U.S. customers only. For international customers, an export license is required. If you are interested in obtaining this plasmid, please contact [email protected]

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-SARS-Spike was a gift from Fang Li (Addgene plasmid # 145031 ; http://n2t.net/addgene:145031 ; RRID:Addgene_145031)

  • For your References section:

    Structural basis of receptor recognition by SARS-CoV-2. Shang J, Ye G, Shi K, Wan Y, Luo C, Aihara H, Geng Q, Auerbach A, Li F. Nature. 2020 Mar 30. pii: 10.1038/s41586-020-2179-y. doi: 10.1038/s41586-020-2179-y. 10.1038/s41586-020-2179-y PubMed 32225175
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