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TMPRSS2
(Plasmid #53887)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53887 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *
Cloning Grade DNA 53887-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $95 *

* Login to view industry pricing.

Backbone

  • Vector backbone
    pCSDest
  • Backbone manufacturer
    Lawson Lab (Addgene Plasmid 22423)
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TMPRSS2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1479
  • Entrez Gene
    TMPRSS2 (a.k.a. PRSS10)
  • Promoter SP6 and CMV

Cloning Information

Resource Information

Depositor Comments

NM_005656

Information for Cloning Grade DNA (Catalog # 53887-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TMPRSS2 was a gift from Roger Reeves (Addgene plasmid # 53887 ; http://n2t.net/addgene:53887 ; RRID:Addgene_53887)
  • For your References section:

    Survey of Human Chromosome 21 Gene Expression Effects on Early Development in Danio rerio. Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, McCallion AS, Katsanis N, Reeves RH. G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 10.1534/g3.118.200144. 10.1534/g3.118.200144 PubMed 29760202