Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

EID1-pGex-2TK
(Plasmid #22689)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22689 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGex2T
  • Backbone manufacturer
    Pharmacia
  • Backbone size w/o insert (bp) 4938
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha, XL1 etc. for propagation of plasmid, BL21 or derivatives for protein expression
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E1A-like inhibitor of differentiation 1
  • Alt name
    EID1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    574
  • Mutation
    wt cDNA
  • GenBank ID
    NM_014335
  • Entrez Gene
    EID1 (a.k.a. PNAS-22, C15orf3, CRI1, EID-1, IRO45620, MGC138883, MGC138884, PTD014, RBP21)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer pGex5'
  • 3′ sequencing primer pGex3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EID1-pGex-2TK was a gift from William Kaelin (Addgene plasmid # 22689 ; http://n2t.net/addgene:22689 ; RRID:Addgene_22689)
  • For your References section:

    Cells degrade a novel inhibitor of differentiation with E1A-like properties upon exiting the cell cycle. Miyake S, Sellers WR, Safran M, Li X, Zhao W, Grossman SR, Gan J, DeCaprio JA, Adams PD, Kaelin WG. Mol Cell Biol. 2000 Dec . 20(23):8889-902. 10.1128/MCB.20.23.8889-8902.2000 PubMed 11073989