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qs5
(Plasmid #24498)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24498 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 24498-D50 50 μg of DNA in Tris buffer $495
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DNA 24498-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA1
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    Plasmid contains SupF. Grow in TOP10/P3 cells.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Gq alpha
  • Alt name
    G protein alpha subunit
  • Alt name
    Gnaq
  • Species
    M. musculus (mouse)
  • Mutation
    Replacement of the five carboxyl-terminal amino acids of Gq alpha (EYNLV) with those of Gs alpha(QYELL)
  • Entrez Gene
    Gnaq (a.k.a. 1110005L02Rik, 6230401I02Rik, Dsk1, Dsk10, Galphaq, Gq, GqI)
  • Tag / Fusion Protein
    • HA (see comments)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NsiI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gs alpha residues (EYNLV to QYELL). This construct allows some Gs-coupled receptors to stimulate phospholipase C.

Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling.

Please see supplemental document for G-protein chimera user manual.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    qs5 was a gift from Bruce Conklin (Addgene plasmid # 24498 ; http://n2t.net/addgene:24498 ; RRID:Addgene_24498)

  • For your References section:

    Carboxyl-terminal mutations of Gq alpha and Gs alpha that alter the fidelity of receptor activation. Conklin BR, Herzmark P, Ishida S, Voyno-Yasenetskaya TA, Sun Y, Farfel Z, Bourne HR. Mol Pharmacol. 1996 Oct . 50(4):885-90. PubMed 8863834
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