Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24499||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Tetracycline
Growth instructionsPlasmid contains SupF. Grow in TOP10/P3 cells.
Gene/Insert nameGs alpha
Alt nameG protein alpha subunit
SpeciesR. norvegicus (rat)
MutationThis is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV).
Entrez GeneGnas (a.k.a. ALEX, G-alpha-8, Gnas1, Gnpas, Nesp55, SCG6)
/ Fusion Protein
- HA (see comments)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV). This construct allows some Gq-coupled receptors to stimulate Adenylate cyclase. There isn't much experience with this chimera at the moment. It may be useful for people who find the AC stimulation is better readout of receptor activation than PLC stimulation.
Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:sq5 was a gift from Bruce Conklin (Addgene plasmid # 24499 ; http://n2t.net/addgene:24499 ; RRID:Addgene_24499)
For your References section:Carboxyl-terminal mutations of Gq alpha and Gs alpha that alter the fidelity of receptor activation. Conklin BR, Herzmark P, Ishida S, Voyno-Yasenetskaya TA, Sun Y, Farfel Z, Bourne HR. Mol Pharmacol. 1996 Oct . 50(4):885-90. PubMed 8863834