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sq5
(Plasmid #24499)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24499 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    Plasmid contains SupF. Grow in TOP10/P3 cells.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Gs alpha
  • Alt name
    G protein alpha subunit
  • Alt name
    GNAS
  • Species
    R. norvegicus (rat)
  • Mutation
    This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV).
  • Entrez Gene
    Gnas (a.k.a. ALEX, Gnas1, Gnpas, Nesp55, SCG6)
  • Tag / Fusion Protein
    • HA (see comments)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV). This construct allows some Gq-coupled receptors to stimulate Adenylate cyclase. There isn't much experience with this chimera at the moment. It may be useful for people who find the AC stimulation is better readout of receptor activation than PLC stimulation.

Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling.

Please see supplemental document for G-protein chimera user manual.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sq5 was a gift from Bruce Conklin (Addgene plasmid # 24499)
  • For your References section:

    Carboxyl-terminal mutations of Gq alpha and Gs alpha that alter the fidelity of receptor activation. Conklin BR, Herzmark P, Ishida S, Voyno-Yasenetskaya TA, Sun Y, Farfel Z, Bourne HR. Mol Pharmacol. 1996 Oct . 50(4):885-90. PubMed 8863834