Plasmid 24499: sq5

• Gene/insert name: Gs alpha
• Alt name: G protein alpha subunit
• Alt name: GNAS
• Species: R. norvegicus (rat)
• Entrez Gene: Gnas (Gnas1, Gnpas, Nesp55)
  
• Fusion protein or tag: HA (see comments)
• Mutation: This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV).
• Vector backbone: pcDNA1
  (Search Vector Database)
• Vector type: Mammalian Expression
• Backbone size w/o insert (bp): 4000
• Cloning site 5': BglII
• Site destroyed during cloning: Unknown
• Cloning site 3': XhoI
• Site destroyed during cloning: Unknown
• 5' sequencing primer: SP6
• Bacterial resistance(s) Ampicillin and Tetracycline
• Growth strain(s) Top10/P3
• Growth temperature (℃): 37
• Growth instructions: Plasmid contains SupF. Grow in TOP10/P3 cells.
• High or low copy: Unknown
• Sequence: View sequences (2)
• Supplemental document: G-protein chimera user manual (application/pdf)
• Principal Investigator: Bruce Conklin
• Terms and Licenses: MTA

Comments: 

This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV). This construct allows some Gq-coupled receptors to stimulate Adenylate cyclase. There isn't much experience with this chimera at the moment. It may be useful for people who find the AC stimulation is better readout of receptor activation than PLC stimulation.

Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling.

Please see supplemental document for G-protein chimera user manual.

Article: Carboxyl-terminal mutations of Gq alpha and Gs alpha that alter the fidelity of receptor activation. Conklin et al (Mol Pharmacol. 1996 Oct . 50(4):885-90. PubMed)