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qs5
(Plasmid #24498)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24498 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    Plasmid contains SupF. Grow in TOP10/P3 cells.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Gq alpha
  • Alt name
    G protein alpha subunit
  • Alt name
    Gnaq
  • Species
    M. musculus (mouse)
  • Mutation
    Replacement of the five carboxyl-terminal amino acids of Gq alpha (EYNLV) with those of Gs alpha(QYELL)
  • Entrez Gene
    Gnaq (a.k.a. 1110005L02Rik, 6230401I02Rik, AA408290, AW060788, Dsk1, Dsk10, Galphaq, Gq, GqI)
  • Tag / Fusion Protein
    • HA (see comments)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NsiI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gs alpha residues (EYNLV to QYELL). This construct allows some Gs-coupled receptors to stimulate phospholipase C.

Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling.

Please see supplemental document for G-protein chimera user manual.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    qs5 was a gift from Bruce Conklin (Addgene plasmid # 24498)
  • For your References section:

    Carboxyl-terminal mutations of Gq alpha and Gs alpha that alter the fidelity of receptor activation. Conklin BR, Herzmark P, Ishida S, Voyno-Yasenetskaya TA, Sun Y, Farfel Z, Bourne HR. Mol Pharmacol. 1996 Oct . 50(4):885-90. PubMed 8863834