Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24498||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Tetracycline
Growth instructionsPlasmid contains SupF. Grow in TOP10/P3 cells.
Gene/Insert nameGq alpha
Alt nameG protein alpha subunit
SpeciesM. musculus (mouse)
MutationReplacement of the five carboxyl-terminal amino acids of Gq alpha (EYNLV) with those of Gs alpha(QYELL)
Entrez GeneGnaq (a.k.a. 1110005L02Rik, 6230401I02Rik, AA408290, AW060788, Dsk1, Dsk10, Galphaq, Gq, GqI)
/ Fusion Protein
- HA (see comments)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site NsiI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SP6 (Common Sequencing Primers)
This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gs alpha residues (EYNLV to QYELL). This construct allows some Gs-coupled receptors to stimulate phospholipase C.
Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:qs5 was a gift from Bruce Conklin (Addgene plasmid # 24498 ; http://n2t.net/addgene:24498 ; RRID:Addgene_24498)
For your References section:Carboxyl-terminal mutations of Gq alpha and Gs alpha that alter the fidelity of receptor activation. Conklin BR, Herzmark P, Ishida S, Voyno-Yasenetskaya TA, Sun Y, Farfel Z, Bourne HR. Mol Pharmacol. 1996 Oct . 50(4):885-90. PubMed 8863834