pAAV_EF1a-FLEx(FRT)-GCaMP8m
(Plasmid
#252530)
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PurposeExpresses the genetically encoded calcium indicator GCaMP8m in Flp-expressing cells under the EF1a promoter for calcium imaging.
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Depositing Lab
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Depositing OrganizationHarvard Medical School
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252530 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV-Ef1a-FLEx(FRT)
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGCaMP8m
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SpeciesSynthetic
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Insert Size (bp)1269
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.21203/rs.3.rs-6831193/v1 for preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_EF1a-FLEx(FRT)-GCaMP8m was a gift from Gordon Fishell (Addgene plasmid # 252530 ; http://n2t.net/addgene:252530 ; RRID:Addgene_252530) -
For your References section:
Feature-specific threat coding in lateral septum guides defensive action. Bhatti Mazo DL, Berger MZC, Pasqualini AL, Wu SJ, Reid CM, Brito SI, Qiu S, Levitt P, Harwell CC, Anthony TE, Fishell G. Nature. 2026 Aug;656(8127):415-424. doi: 10.1038/s41586-026-10520-9. Epub 2026 May 20. 10.1038/s41586-026-10520-9 PubMed 42162422