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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 39865 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepYD1
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Backbone manufacturerinvitrogen
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemonomeric streptavidin
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Alt namestreptavidin rhizavidin hybrid
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SpeciesSynthetic
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Insert Size (bp)357
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Tags
/ Fusion Proteins
- Aga2 (N terminal on backbone)
- HA (N terminal on insert)
- FLAG (C terminal on backbone)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYD1-mSA was a gift from Sheldon Park (Addgene plasmid # 39865 ; http://n2t.net/addgene:39865 ; RRID:Addgene_39865) -
For your References section:
Stable, high-affinity streptavidin monomer for protein labeling and monovalent biotin detection. Lim KH, Huang H, Pralle A, Park S. Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. Epub 2012 Aug 8. 10.1002/bit.24605 PubMed 22806584
