|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||41843||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6142
- Total vector size (bp) 6456
Vector typeYeast Expression
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberLow Copy
Insert Size (bp)183
/ Fusion Proteins
- HA (N terminal on backbone)
- c-myc (C terminal on backbone)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCTcon2 was a gift from Dane Wittrup (Addgene plasmid # 41843 ; http://n2t.net/addgene:41843 ; RRID:Addgene_41843)
For your References section:Isolating and engineering human antibodies using yeast surface display. Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD. Nat Protoc. 2006;1(2):755-68. 10.1038/nprot.2006.94 PubMed 17406305