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pET21-Streptavidin-Glutamate_Tag
(Plasmid #46367)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 46367 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET21
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 5800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Dh5alpha for propagation of plasmid. Bl21[DE3] or similar for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Streptavidin-Glutamate_Tag
  • Alt name
    SAe
  • Species
    E. coli
  • Insert Size (bp)
    408
  • Promoter T7
  • Tag / Fusion Protein
    • 6 glutamate tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7F
  • 3′ sequencing primer T7R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21-Streptavidin-Glutamate_Tag was a gift from Mark Howarth (Addgene plasmid # 46367)
  • For your References section:

    Plug-And-Play Pairing Via Defined Divalent Streptavidins. Fairhead M, Krndija D, Lowe ED, Howarth M. J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 10.1016/j.jmb.2013.09.016. 10.1016/j.jmb.2013.09.016 PubMed 24056174