|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51484||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2400
Modifications to backboneInsertion of HygroR and MCS
Vector typeWorm Expression
Growth in Bacteria
Growth instructionsImportant to use dual selection to limit recombination of att sites.
Copy numberHigh Copy
- Cloning method Unknown
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byHygroR derived from plasmid from Jason Chin's lab (Cambridge, UK). See Radman et al. (2013) publication for details on hygromycin B selection.
Terms and Licenses
See www.wormbuilder.org for updated protocols.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFJ1663 was a gift from Erik Jorgensen (Addgene plasmid # 51484 ; http://n2t.net/addgene:51484 ; RRID:Addgene_51484)