pCFJ90 - Pmyo-2::mCherry::unc-54utr
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19327||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2446
Vector typeWorm Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesC. elegans (nematode)
Insert Size (bp)2670
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Use as co-injection marker. Red mCherry fluorescence in the pharynx. Do not use at high concentration (above 5 ng/ul) as it is toxic to animals.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFJ90 - Pmyo-2::mCherry::unc-54utr was a gift from Erik Jorgensen (Addgene plasmid # 19327 ; http://n2t.net/addgene:19327 ; RRID:Addgene_19327)
For your References section:Single-copy insertion of transgenes in Caenorhabditis elegans. Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, Blake J Newman, Jason M Thumme, Søren-Peter Olesen, Morten Grunnet & Erik M Jorgensen. 10.1038/ng.248 PubMed 18953339