pCFJ104 - Pmyo-3::mCherry::unc-54
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19328||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2416
Vector typeWorm Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesC. elegans (nematode)
Insert Size (bp)4224
/ Fusion Protein
- Cloning method Gateway Cloning
- 5′ cloning site attR4 (destroyed during cloning)
- 3′ cloning site attR3 (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Red mCherry expression in the pharynx driven by the myo-3 promoter. unc-54 utr.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFJ104 - Pmyo-3::mCherry::unc-54 was a gift from Erik Jorgensen (Addgene plasmid # 19328 ; http://n2t.net/addgene:19328 ; RRID:Addgene_19328)
For your References section:Single-copy insertion of transgenes in Caenorhabditis elegans. Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, Blake J Newman, Jason M Thumme, Søren-Peter Olesen, Morten Grunnet & Erik M Jorgensen. 10.1038/ng.248 PubMed 18953339