Addgene - pGH8 - pRAB-3::mCherry::unc-54utr Plasmid Data

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Plasmid 19359: pGH8 - pRAB-3::mCherry::unc-54utr

Gene/insert name:
Prab-3::mCherry
Insert size:
2915
Species:
C. elegans (nematode)
Fusion protein or tag:
mCherry
Vector backbone:
pDESTR4-R3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Worm Expression
Backbone size w/o insert:
2432
Cloning method:
Gateway Cloning
Cloning site 5':
attR4
Site destroyed during cloning:
Yes
Cloning site 3':
attR3
Site destroyed during cloning:
Yes
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Erik Jorgensen
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

Coinjection marker. mCherry expression in the nervous system.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Single-copy insertion of transgenes in Caenorhabditis elegans Christian et al

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19359" in your Materials and Methods section.