Addgene - pCFJ151 - ttTi5605

Plasmid 19330: pCFJ151 - ttTi5605_MCS

Gene/insert name:
ttTi5605 targeting region
Insert size:
5075
Species:
C. elegans (nematode)
Vector backbone:
N/A
(Search Vector Database)
Vector type:
Worm Expression
Backbone size w/o insert (bp):
2334
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Erik Jorgensen
Terms and Licenses:
MTA

Comments:

Standard Multiple Cloning site vector to generate MosSCI inserts at ttTi5605 location. Contains C.Briggsae unc-119 rescue gene. Also contains genomic regions flanking the ttTi5605 Mos1 insertion. Use C.elegans strain EG4322 to generate insertions.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Single-copy insertion of transgenes in Caenorhabditis elegans Christian et al

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19330" in your Materials and Methods section.