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pcDNA3.cytGAP
(Plasmid #78734)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78734 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 78734-D50 50 μg of DNA in Tris buffer $495
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DNA 78734-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cytGAP
  • Species
    Synthetic
  • Promoter CMV
  • Tag / Fusion Protein
    • luciferase (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GAP is a fusion of two Aequorea victoria proteins, GFP and apo-aequorin, with a linker in between. The excitation spectrum of GAP maintained the two peaks of wtGFP, at 403 and 470 nm, and it was sensitive to Ca2+.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.cytGAP was a gift from Teresa Alonso (Addgene plasmid # 78734 ; http://n2t.net/addgene:78734 ; RRID:Addgene_78734)

  • For your References section:

    GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in organelles. Rodriguez-Garcia A, Rojo-Ruiz J, Navas-Navarro P, Aulestia FJ, Gallego-Sandin S, Garcia-Sancho J, Alonso MT. Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2584-9. doi: 10.1073/pnas.1316539111. Epub 2014 Feb 5. 10.1073/pnas.1316539111 PubMed 24501126
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