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pCasperAUG-Gal4-X
(Plasmid #8378)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8378 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCasper
  • Backbone manufacturer
    Public Domain--Carl Thummel
  • Backbone size w/o insert (bp) 9000
  • Vector type
    Drosophila P-element vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Yeast Gal4
  • Species
    S. cerevisiae (budding yeast); fused to first ~30 amino acids of Drosophila ADH
  • Insert Size (bp)
    3000
  • GenBank ID
    K01486
  • Entrez Gene
    GAL4 (a.k.a. YPL248C, GAL81)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI, Asp718I*, NotI, BamHI, BglII, PmeI, Asp718* (not destroyed)
  • 5′ sequencing primer gggttttattaacttacat
  • 3′ sequencing primer taagtcggcaaatatcgc
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Plasmid was constructed by deleting B-Gal gene from pCasperAUG-Bgal from Carl Thummel/Univ. of Utah and replacing with the coding region of Gal4 from the GL83 plasmid provided by Gary Struhl/Columbia University
  • Terms and Licenses
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCasperAUG-Gal4-X was a gift from Leslie Vosshall (Addgene plasmid # 8378)
  • For your References section:

    An olfactory sensory map in the fly brain. Vosshall LB, Wong AM, Axel R. Cell 2000 Jul 21;102(2):147-59. 10.1016/S0092-8674(00)00021-0 PubMed 10943836