Addgene - pCasperAUG-Gal4-X Plasmid Data

Plasmid 8378: pCasperAUG-Gal4-X

Gene/insert name:
Yeast Gal4
Insert size:
3000
Species:
S. cerevisiae (budding yeast)
Species:
fused to first ~30 amino acids of Drosophila ADH
GenBank ID:
K01486
Entrez Gene:
GAL4 (YPL248C, GAL81)
Vector backbone:
pCasper
(Search Vector Database)
Backbone manufacturer:
Public Domain--Carl Thummel
Vector type:
Drosophila P-element vector
Backbone size w/o insert (bp):
9000
Cloning site 5':
EcoRI, Asp718I*, NotI, BamHI, BglII, PmeI, Asp718*
Site destroyed during cloning:
No
5' sequencing primer:
gggttttattaacttacat List of Sequencing Primers
3' sequencing primer:
taagtcggcaaatatcgc
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
Plasmid was constructed by deleting B-Gal gene from pCasperAUG-Bgal from Carl Thummel/Univ. of Utah and replacing with the coding region of Gal4 from the GL83 plasmid provided by Gary Struhl/Columbia University
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Leslie Vosshall
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An olfactory sensory map in the fly brain. Vosshall et al (Cell 2000 Jul 21;102(2):147-59. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8378" in your Materials and Methods section.