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Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases.
Citorik RJ, Mimee M, Lu TK
Nat Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
61270pMM178Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.
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61271pMM441Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
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61272pRC319Also referred to as pÎŚRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.
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