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Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Nielsen AA, Voigt CA
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
PubMed

Plasmids from Article

ID Plasmid Purpose  
62244pAN-PTet-dCas9controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.
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62245pAN-PTet-YFPused to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.
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62246pAN-PBAD-YFPused to characterize the promoter activities of PBAD for dCas9 and sgRNA response functions.
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62247pAN-PA1-RFPreporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1
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62248pAN-PA2-RFPreporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1
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62249pAN-PA3-RFPreporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1
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62250pAN-PA4-RFPreporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1
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62251pAN-PA5-RFPreporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1
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62255pAN-PBAD-sgRNA-A1NTexpression of A1NT sgRNA from the arabinose-inducible promoter
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62256pAN-PBAD-sgRNA-A1Texpression of A1T sgRNA from the arabinose-inducible promoter
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62257pAN-PBAD-sgRNA-A2NTexpression of A2NT sgRNA from the arabinose-inducible promoter
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62258pAN-PBAD-sgRNA-A2Texpression of A2T sgRNA from the arabinose-inducible promoter
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62259pAN-PBAD-sgRNA-A3NTexpression of A3NT sgRNA from the arabinose-inducible promoter
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62260pAN-PBAD-sgRNA-A3Texpression of A3T sgRNA from the arabinose-inducible promoter
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62261pAN-PBAD-sgRNA-A4NTexpression of A4NT sgRNA from the arabinose-inducible promoter
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62262pAN-PBAD-sgRNA-A4Texpression of A4T sgRNA from the arabinose-inducible promoter
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62263pAN-PBAD-sgRNA-A5NTexpression of A5NT sgRNA from the arabinose-inducible promoter
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62264pAN-PBAD-sgRNA-A5Texpression of A5T sgRNA from the arabinose-inducible promoter
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62285pAN-PBAD-sgRNA-scrambleexpression of scramble sgRNA from the arabinose-inducible promoter
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62286pAN-PBAD-sgRNA-VRexpression of VR sgRNA from the arabinose-inducible promoter
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62306pAN-NORsynthetic circuit: NOR gate
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62307pAN-ORsynthetic circuit: OR gate
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62308pAN-ANDsynthetic circuit: AND gate
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62309pAN-OR-MalT-3NTsynthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genome
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