IRES-GFP FoxG1
(Plasmid #14935)

• Depositing Lab: Joan Massague
• Publication: Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Backbone

• Vector backbone: pIRES2-EGFP
• Backbone manufacturer: BD Biosciences
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FoxG1
• Species: H. sapiens (human)
• Entrez Gene: FOXG1 (a.k.a. BF1, BF2, FHKL3, FKH2, FKHL1, FKHL2, FKHL3, FKHL4, FOXG1A, FOXG1B, FOXG1C, HBF-1, HBF-2, HBF-3, HBF-G2, HBF2, HFK1, HFK2, HFK3, KHL2, QIN)
• Tag / Fusion Protein:
  • IRES-GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that a nucleotide BLAST of the insert sequence returns a match to GenBank: BC035020.2.