pMXs-hc-MYC
(Plasmid #17220)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pMXs
  • Backbone size w/o insert (bp) 4600
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    c-MYC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1320
  • Mutation
    None
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site attB1 (not destroyed)
  • 3′ cloning site attB2 (not destroyed)
  • 5′ sequencing primer pMX-S1811
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
  • Terms and Licenses

Depositor Comments

The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-hc-MYC was a gift from Shinya Yamanaka (Addgene plasmid # 17220)
  • For your References section:

    Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Cell. 2007 Nov 30. 131(5):861-72. 10.1016/j.cell.2007.11.019 PubMed 18035408