Addgene - pLenti6/UbC/mSlc7a1 Plasmid Data

Plasmid 17224: pLenti6/UbC/mSlc7a1

Gene/insert name:
Slc7a1
Insert size:
1870
Species:
M. musculus (mouse)
Entrez Gene:
Slc7a1 (4831426K01Rik, AI447493, Atrc-1, Atrc1, Cat1, Rec-1, Rev-1, mCAT-1)
Mutation:
None
Vector backbone:
pLenti6/UbC/V5-DEST
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
7600
Cloning method:
Gateway Cloning
Cloning site 5':
attB1
Site destroyed during cloning:
No
Cloning site 3':
attB2
Site destroyed during cloning:
No
5' sequencing primer:
UbC forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
We recommend using Stbl3 E. coli strain (Invitrogen).
High or low copy:
High Copy
Selectable markers:
Blasticidin
Sequence: View sequences (1)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi et al (Cell. 2007 Nov 30. 131(5):861-72. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17224" in your Materials and Methods section.