Addgene - pMXs-Klf4 Plasmid Data

Plasmid 13370: pMXs-Klf4

Gene/insert name:
Kruppel-like factor 4 (gut)
Alt name:
Klf4
Insert size:
1425
Species:
M. musculus (mouse)
Entrez Gene:
Klf4 (RP23-322L22.2, EZF, Gklf, Zie)
Mutation:
None
Vector backbone:
pMXs
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
4600
5' sequencing primer:
pMX-S1811 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA

Comments:

The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.

Mouse Klf4 includes G372A, A459C, G527C, G633C, G1021C, C1305G and T1368C mutations.
All of substitutions can be found in EST database of NCBI. So we believe that these differences are caused by SNPs.
As we have shown in the previous our papers, these changes do not affect biological functions of the transcription factors during reprogramming.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi et al (Cell. 2006 Aug 25. 126(4):663-76. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13370" in your Materials and Methods section.