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pMXs-Oct3/4
(Plasmid #13366)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13366 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMXs
  • Backbone size w/o insert (bp) 4600
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    POU domain, class 5, transcription factor 1
  • Alt name
    Oct3/4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1134
  • Mutation
    None
  • Entrez Gene
    Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.

Mouse Oct3/4 includes C91T mutation.
We found that EST clones including substitution C91 to T in NCBI database. So we believe that these substitutions are ascribed to SNPs.
CJ069184 RIKEN full-length enriched mouse cDNA library, CRL-2070
CJ069414 RIKEN full-length enriched mouse cDNA library, CRL-2070
As we have shown in the previous our papers, these changes do not affect biological functions of the transcription factors during reprogramming.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-Oct3/4 was a gift from Shinya Yamanaka (Addgene plasmid # 13366)
  • For your References section:

    Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174