Addgene - pMXs-Sox2 Plasmid Data

Plasmid 13367: pMXs-Sox2

Gene/insert name:
SRY-box containing gene 2
Alt name:
Sox2
Insert size:
960
Species:
M. musculus (mouse)
Entrez Gene:
Sox2 (AL606746.1, Sox-2, lcc, ysb)
Mutation:
None
Vector backbone:
pMXs
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
4600
5' sequencing primer:
pMX-S1811 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA

Comments:

The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.

The Sox2 cDNA contains a modified ATG start codon to TTG for cloning into vectors with an N-terminal tag. If
this cDNA is used in a non-tagged construct, a functional protein (missing the first 3 amino acids--MYN) is produced as confirmed by co-immunoprecipitation, reporter assay, and
other experiments.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi et al (Cell. 2006 Aug 25. 126(4):663-76. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13367" in your Materials and Methods section.