ZP45 Prickle-1
(Plasmid #16897)

• Depositing Lab: Randall Moon
• Publication: Zebrafish Probe Plasmids (unpublished)

Backbone

• Vector backbone: pGEM-T easy
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Prickle-1
• Alt name: pk1
• Species: D. rerio (zebrafish)
• Entrez Gene: prickle1a (a.k.a. fk71b09, pk1, prickle1, wu:fk71b09)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full length prickle-1 with 42bp of 5' UTR, cloned into pGEM-Teasy. The construct makes good in situ probes but does not translate in vitro due to an atg in the polylinker.

To make antisense probe: cut with Nco1, transcribe with SP6. For the sense strand cut with Nde1 and transcribe with T7.

Citation: Michael T. Veeman et al., Current Biology 13: 680-685 (2003).